Enviro-One Antibacterial Efficacy Testing Results

An independent study done by Biological Consulting Services of North Florida, inc. have been conducted and concludes that Enviro-One Formula effectively removes 99% of Escherichia coli (E. coli) and Salmonella typhimurium. See below for test results.

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Biological Consulting Services

of North Florida, Inc.

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March 6, 2007

RE: Antibacterial Efficacy Testing of Formula 080 (sold as Enviro-One Multi-Use Green Ceaner.) Liquid Soap (Lot# 011102-05).

Dear Ms. DíAire: Greetings.

We have conducted the antimicrobial efficacy study that we proposed previously. Following, you will find our report on the analysis that was conducted.

Should you have any questions please do not hesitate to contact me.

Sincerely,

George Lukasik, Ph.D. Sr. Laboratory Director

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Analysis Parameter: Bacterial killing efficacy of Formula 080 (sold as Enviro-One Multi-Use Green Cleaner) liquid soap (Lot# 011102-05).

Four bottles of Formula 080 (sold as Enviro-One Multi-Use Green Cleaner) liquid soap (Lot#011102-05) were delivered to our laboratory on February 23 ,2007

Methodology: Analysis was conducted as per the proposal submitted on February 20th, 2007 and the following phone conversations with Ms. DíAire. Briefly, the bacteria Escherichia coli C3000 (ATCC 15597), and Salmonella typhimurium were grown up overnight in Tryptic Soy Broth (Beckton Dickinson, MD) at 37°C the day prior to the experiment. Dilutions of the supplied soap stock solution were prepared in standard reagent grade water as follows: 1:5, 1:10, 1:100, and reagent water was used as the negative control. Aliquot (0.01 ml) of each of the bacterial overnight cultures was added to 100 ml of the above prepared solutions. The solutions were agitated on an orbital shaker on low speed for 5 minutes and one hour. At the previously specified time intervals, aliquots of the solutions were removed and the number of bacteria (as colony forming units; cfu) present in each solution was determined by spread plating onto MacConkey Agar (Beckton Dickinson, MD). All assays were performed in duplicates. In The following table you will find the summary of the results

 

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Table 1. Efficacy of Formula 080 (sold as Enviro-One Multi-Use Green Cleaner) liquid soap (Lot# 011102-05) on the inactivation of Escherichia coli C3000 (ATCC 15597), and Salmonella typhimuriumafter 5 and 60 minutes of agitation.

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Treatment

Escherichia coli C3000

Salmonella typhimurium

Average cfu/ml*

Efficacy (percent reduction as compared to water control)

Average cfu/ml*

Efficacy (% reduction as compared to water control)

1:5
5 minutes

<5.0 x 102

>99%

<5.0 x 102

>99%

1:10
5 minutes

<5.0 x 102

>99%

<5.0 x 102

>99%

1:100
5 minutes

1.9 x 104

74.3%

5.6 x 104

70.5%

Water (Control)
5 Minutes

7.4 x 104

Control

1.9 x 105

Control

1:5
60 minutes

<1.0 x 102

>99%

<1.0 x 102

>99%

1:10
60 minutes

<1.0 x 102

>99%

<1.0 x 102

>99%

1:100
60 minutes

3.1 x 104

54.4%

4.1 x 104

65.8%

Water (Control)
60 Minutes

6.8 x 104

Control

1.2 x 105

Control

*cfu/ml = Colony Forming units of bacteria per milliliter of seeded soap/water solution

BCS Laboratories, Inc.- Gainesville
4641 NW 6th Street, Suite C, Gainesville, Florida 32609
Tel. (352) 377-9272, Fax. (352) 377-5630
FL DOH Laboratory #E82924, EPA# FL01147
www.microbioservices.com